Abstract:
Oriental tobaccos in the Republic of Macedonia are selected according to classical methods,
which is quite a long process. Today, one of the most commonly used biotechnological methods
in plants is the doubled haploid method, whose main purpose is to shorten selection processes in
field conditions, while obtaining homozygous lines in the first generation. Tobacco is an ideal
plant for the production of haploid plants, tobacco crops produce an explosion of haploids that
are being used today in the process of tobacco hybridization. Dihaploidization was performed by
direct and rogenesis of haploid tobacco plants obtained in the laboratory in vitro at the Scientific
Tobacco Institute - Prilep, using the tissue culture method. Four dihaploid lines of tobacco were
included in the research, as follows: P 146-7 / 1 DH, Jk.l. 301/23DH, Hybrid 301 / n DH, Jk.l.75-
301 DH and their analogues (P 146-7 / 1 Ø, Hybrid 301/23 Ø, Hybrid 301 / n Øand Jk.l.75-301
Ø).Materiality as an expression of the quality of the leaf tissue varies from 25.96 g/m² (Hybrid
301 /n DH) to 36.91 g/m² (Jk.l. 301/23 Ø).The leaf thickness of the tested dihaploid lines and
tobacco varieties is within the range from 76.00 µm (Hybrid 301 / n DH) to 134.50 µm (Hybrid.
301/23 Ø) |
