Abstract:
Steel slag and rice husk bokashi are the ameliorant that expected can decrease P-retention
Phytoremediation represents an effective, low-cost, environmentally friendly alternative that
improves soil properties, although there have been significant developments in the last decade.
From the scientific point of life, the main challenge is to decipher the metabolic pathways
involved in response to contaminants and to understand their regulation. This information is
essential if we aspire to improve the natural capacities of some plant species to remediate
contaminated soils. A comprehensive study of the molecular response to polychlorinated
biphenyls (PCBs), a family of persistent organic pollutants of particular relevance on a global
scale, was obtained. A transcriptomic approach based on RNA-seq technology has been used to
identify the genes involved in the metabolism of in-plant compounds and to quantify their
activation levels in different controlled situations. It is surprising that this response is virtually
unknown at the molecular level, despite its great potential applied in the context of
phytoremediation technology. We apply to hybrid poplar (Populus tremula x alba) crops with
Aroclor 1221. And we took samples of RNA, thus generating a matrix of four elements with
their corresponding controls. On the other hand, we perform functional analyzes with
bioinformatic tools based on sequence comparisons and gene co-expression networks. The
response of genes of particular interest was validated using qRT-PCR technology. This is the
first comprehensive study of the response of a plant organism to the presence of PCBs. A
considerable number of structural and regulatory genes have been found, defining new
transcription factors whose expression is proportional to the concentration of pollutant in the
medium or the time of exposure to it. Correlation analyzes allow us to state that the metabolic
response to PCBs, including possible degrading pathways, is involved in at least fifteen
transcription factors and approximately forty proteins or enzymes that are particularly induced.
|
