Authors:
AI Wenxiang, WU Rong, CHAI Guofei, LIU Ange, QIN Yihang, LIU Runhan, HE Xin, JIANG Xuewei, DAI
Lingyan* ,China |
Abstract:
The quality and yields of Sorghum bicolor (L.) plants are seriously affected by saline-alkali
conditions. VIP, and NAC (NAM, ATAF, and CUC) transcription factors are specific, and have
various functions in plant development and response to various stresses. To study the tolerance
to saline-alkali stress of transgenic sorghum overexpressed by AtVIP1 and GsNAC2 genes,
sorghum seeds were treated with stress solution (NaHCO3: Na2CO3 = 5: 1, 75 mM, pH 9.63).
After the stress treatment, the growth morphological indexes, stress-resistant physiological
indexes and stress-related gene expression levels of sorghum buds were measured. The results
show that the transgenic sorghum with overexpressing AtVIP1 and GsNAC2 genes had a higher
bud length, fresh weight, and moisture content. Compared to the control, lower H2O2 and O2-
levels, relative permeability of the plasma membrane, and MDA content, with higher POD,
CAT, and SOD activities were found in transgenic sorghum. Gene expression analysis revealed
that several stress response genes were up-regulated. Furthermore, these results suggest that
AtVIP1 and GsNAC2 gene play potentially important roles in response to saline-alkali stress,
and may be used in breeding new varieties to improve sorghum yields under adverse
environmental conditions.
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