Abstract:
Biodegradation of petroleum hydrocarbons in a land-treatment bioremediation system requires
an effective degrading microbes and optimum system conditions. The study aimed to obtain the
characteristics of the interaction between the petrophylic consortium (Pseudomonas sp. and
Aspergillus sp.) and the amendment of chicken manure and to obtain the optimum dose to
increase the biodegradation rate of petroleum hydrocarbons, soil pH change, and increased the
population petrophylic fungi. The experiment was carried out on a greenhouse scale, using a
factorial randomized block design (RBD) with two factors and three replications. The first factor
was the inoculation of the petrophylic consortium consisting of: (i) without Petrophylic, (ii) 1%
Petrophylic, (iii) 3% Petrophylic, (iv) 5% Petrophylic. The second factor was the amendment of
chicken manure, consisting of 3 levels: (i) without chicken manure, (ii) 2% chicken manure, and
(iii) 4% chicken manure. The results showed that petrophylic consortium inoculation and
chicken manure amendment did not show an interaction effect on increasing the rate of
biodegradation of petroleum hydrocarbons, soil pH, and increasing the population of petrophylic
fungi. Likewise, the effect of independent treatment could not increase the biodegradation rate
and soil pH.But, the application of chicken manure increased growth of the petrophylic fungus
population during the 4-week incubation time. However, increasing Petrophylic fungi population
density did not show increasing in degrading petroleum hydrocarbons. The dose of chicken
manure used has not been able to supply N and P nutrients to achieve optimal C: N: P ratio
conditions for degrading hydrocarbons effectively, resulting the inoculated petrophylic could not
degrade hydrocarbons maximally. Compatibility between consortium microbes members as a
hydrocarbon-degrading agent is an important factor for achieving effectively bioremediation
performance.
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