Authors:
Pujawati Suryatmana*, Rizky Fadillaha, Ardy Berton C L, Nadia Nuraniya Kamaluddin and Mieke Rochimi
Setiawati ,Indonesia
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Abstract:
Inoculation of Azotobacter sp. and Pseudomononas sp. as a biostimulant agent is an appropriate
strategy to be carried out to improve the phytoremediation performance of petroleum waste.
Azotobacter sp. can produce phytohormones and fix N2, while Pseudomonas sp. are phosphate
solubilizing bacteria. The study aims to examine the potential of Azotobacter sp. and
Pseudomonas sp. in increasing the biodegradation of petroleum hydrocarbons, in the
Phytoremediation system of Sorghum (Sorghum bicolor L.). The research design used was a
factorial randomized block design, which consisted of 2 factors, namely the treatment of
Azotobacter sp., which consists of 4 levels and treatment of Pseudomonas sp. which consists of 4
levels. The results showed that there was an interaction between Azotobacter sp. and
Pseudomonas sp. on the efficiency of hydrocarbon biodegradation, but there was no interaction
to the population of Azotobacter sp. and Pseudomonas sp., and growth of sorghum plants.
Treatment of 2% Azotobacter sp. + 3% Pseudomonas sp. and treatment of 3% Azotobacter sp. +
3% Pseudomonas sp showed the value of biodegradation efficiency which tends to be higher
than other treatments. Aplication of the two isolates was not able to increase the population of
Azotobacter spp, Pseudomonas spp. and the height of sorghum, but can act as a biostimulant to
significantly increase the biodegradation process of petroleum hydrocarbons. |
