Abstract:
This study provides a scientific information about the aqueous and methanol extracts of Prunella
vulgarisa based on its antimicrobial potential against gram positive and gram negative bacteria
isolated from wound infection using the broth dilution and well diffusion method. Results of this
study indicate the presence of many phytochemicals which have antimicrobial activity against
broad spectrum of bacteria. The methanol extract of P. vulgarisa showed highest activity than
aqueous ones. The minimum inhibitory concentration (MIC) of the aqueous extract on the tested
organisms was 25-100mg/ml while in the methanol extract ranged between 25-50mg/ml on the
tested organisms and the minimum bacterial concentration (MBC) of the aqueous extract was 25-
100 mg/ml while the methanol extract ranged between 25-100 mg/ml. The highest activity of
methanol extract demonstrated at 100 Co, 121 Co against S.aureus, K.spp, A.hydrophila, and
S.marcescens, while there was low activity against S.dysenteria and E.coli. The activity of plant
extract increased at acidic pH5-3 whearus, there are slightly increased of plant extract at alkaline
pH 8. P. vulgarisa contained. essential element (Pb, Na, K, Ca, Fe, Zn, P, Mn, Co and Cu) at
different concertation.
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