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ISSN : 2456-8643

Title:
IN VITRO EXAMINATION THE EFFECTS OF SOME FUNGICIDES ON THE BIOCONTROL AGENT TRICHODERMA HARZIANUM – CONTRIBUTION TO THE COMPATIBILITY IN TOBACCO PRODUCTION

Authors:
Biljana Gveroska ,Macedonia

Abstract:
One of the powerful mechanisms of the biocontrol activity of the genus Trichoderma is ? tolerance to pesticides, and their synergistic effect with fungicides is also pointed. The use of these properties, in particular, compatibility in specific production conditions would contribute to increasing their efficacy. The aim of these studies was to determine the influence of the most commonly used active ingredients in tobacco production on the biocontrol agent Trichoderma. hazianum, which is distinguished by its biocontrol activity to numerous pathogenic fungi that cause tobacco diseases. Effects of fungicides were performed on 12 fungicides currently used in tobacco production and one combination used in recomended concentration. The study wasperformed three times using the medium poisoning method, in five replication of the each variant. Most of the fungicides (systemic, contact and the combination) showed a strong as well as significant reducing effecton T. harzianum on the fourth day. Only Proplant 722 SL has a moderate reducing effect. The strong reducing effect of most of the fungicides was completely lost, i.e. on the tenth day they did not affect the development at all. Folicur EW -250, Top M as well as the combination Top M + Proplant 722 SL show strong reducing effect. Therefore, they are not compatible with T. harzianum at all. High compatibility has been found in the Proplant 722 SL, Signum 33WG, Armetil M and Agrizole 250-EC, as well as in the contact fungicides Manfil 80 WP and Enervin WG. The high compatibility of the fungicide after 10 days is a very useful method of application of the biocontrol agent in the fungicidal treatments. Specifically, the BCA can be applied after the expiration of that period, which is actually applicable in practice. Developing an integrated disease management strategy by combing Trichoderma and fungicides to prevent pathogen from goaling resistance and building up Trichoderma population levels in the soil that will be effective would be the goal in tobacco protection from diseases.

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