Authors:
Khoirul Bariyyah, Yusmia Widiastuti, Putri Istianingrum, Fathurrahman, Sri Hartatik and Didik Puji Restanto ,Indonesia |
Abstract:
Tissue culture is an alternative to propagate the seeds of Red Durian uniformly and in great quantity. Type of explants isone of important factors that affects on callus induction. The callus will be used to form somatic embryogenesis. Objective of the research was to find out the explants type, which is able to form callus. This research used single factor, the explants. Explants used in this research include leaf, stem, and seed. The explants is grown in MS medium by adding ZPT 2,4 D 200 ppm, Picloram 0.5 mg/L. Results of the research showed that the highest contamination level was on explants, which derived from stem 100%, and leaf 70% with fungi as the contaminant. Explants which are able to produce shoot, are derived from seed explants. The age of the callus is 25 HSK, yellowish white with a solid callus structure |
