Abstract:
The ubiquitous Salmonellae are one of the most important causes of human foodborne infection.
In fact, avian species can be infected either by non-motile Salmonellae (Salmonella gallinarum
and Salmonella pullorum) leading to fowl typhoid and pullorum disease, and motile Salmonellae
(Non-typhoid group: NTG) causing salmonellosis in chicken and having a zoonotic impact. The
present study was conducted from March 2015 to May 2017 in 72 laying hen farms located in
different areas in Morocco. In order to determine the prevalence of Salmonella spp.
Bacteriological investigations, Salmonella Phage test (SPT) and Polymerase chain reaction to
detect invA gene were carried out. A specific PCR to reveal spvC plasmid virulence gene was
performed. Bacteriological investigations and SPT revealed that 34.72% (25/72) of sampled
farms were infected by Salmonellaspp. Otherwise, invA gene was detected in 24; all of
Salmonellaisolates carry spvC virulence plasmid gene. According to the serotyping of 25
Salmonella isolates, 13 were detected as SalmonellaGalinarum, 6 as S. Enteritidis and 6 others
were recognized as S.Cyprus, S.Kottbus, S.Curacao, S.Fayed, S.Chomedey, S.Chailley.Finally,
we can conclude that avian salmonellosis still an important infection which could have a
negative impact on poultry production and also on public health |
