Authors:
Mohammad Zainul Fadli
, Mudawamah Mudawamah, Irawati Dinasari Retnaningtyas
, Gatot Ciptadi and
Oktavia Rahayu Puspitarini ,Indonesia |
Abstract:
In Indonesia, rabbits have developed as small-scale businesses in tourist areas in the form of
ornamental rabbits or meat rabbits. The rabbits are kept various kinds of purebred or
crossbreeding between purebred and local Indonesian rabbits. The purpose of this study was to
observe the genotype F1 crossbreed through the molecular analysis of various rabbit breeds that
had been carried out by rabbit breeders as genotype diversity. This research method was a case
study and experiment using various rabbits that many farmers raised. The purebred of rabbits
used include Rex, Satin, Lion, Flemish Giant, New Zealand White, local Indonesian rabbits. The
rabbit crossed include Rex with Satin, Lion with Indonesian Local-bred, Flemish Giant with
Local. The Phenotype analysis for F1 derived from purebred and Local-bred under the same
management. Molecular analysis was carried out using Polymerase Chain Reaction (PCR) and
Polymerase Chain Reaction-Restricted Fragment Length Polymorphism (PCR-RLPF) with GH
primers with HaeIII restriction enzymes. Descriptive analyzed was used for describing research
data. The results showed that all samples carried out PCR with GH5 were amplified at 289 bp.
PCR results showed that almost all local rabbits were AB-type (85% of the total sample) while
all rabbits were purebred and F1 crossbreed with Local-bred had BB type, and phenotype
superiority F1 to the dam was 18.51-7.41 %. The conclusion from this study was the F1 derived
from purebreds, and Local-bred had a superiority genotype and phenotype compared to the dams
(Local-bred).
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