Authors: JingjingLi , Tian Wang , Yuantong Li ,Yuanyuan Li and Dongsheng Wang ,China |
Abstract: Actinomyceteis a kind of microbial resources with great medicinal value. Plant rhizosphere soils
act as important sites for the survival of actinomycetes. Actinomycetes can help protect the root
of plants from pathogenic bacteria infection and some actinomycetes have the ability to produce
enzymes and antimicrobial compounds. We studied the antagonistic and enzyme-producing
activities of actinomycetes isolated from rhizosphere soil of PaeconiaJishanensis in Yuncheng
City, China, so as to provide the scientific basis for the utilization and protection of this
endangered plant species. About 52actinomycete isolates were isolated usingserial dilution and
spread-plate techniques. The antagonistic activity of actinomycete isolates was assayed using an
agar block method against 4 typical bacterial and fungal species. The protease and amylase
activities were detected by the transparent plate method. The results show that 11isolates showed
antibacterial activity and 20 isolates showed protease and amylaseactivity. In conclusion,
rhizosphere soil of PaeconiaJishanensis possesses a high diversity of actinomycetes and serves as
a natural source of bioactive compound-producing actinomycetes. |