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ISSN : 2456-8643

Title:
ISOLATION AND BIOACTIVITY ASSAY OF ACTINOMYCETESIN RHIZOSPHERE OF PAEONIAJISHANENSIS

Authors:
JingjingLi , Tian Wang , Yuantong Li ,Yuanyuan Li and Dongsheng Wang ,China

Abstract:
Actinomyceteis a kind of microbial resources with great medicinal value. Plant rhizosphere soils act as important sites for the survival of actinomycetes. Actinomycetes can help protect the root of plants from pathogenic bacteria infection and some actinomycetes have the ability to produce enzymes and antimicrobial compounds. We studied the antagonistic and enzyme-producing activities of actinomycetes isolated from rhizosphere soil of PaeconiaJishanensis in Yuncheng City, China, so as to provide the scientific basis for the utilization and protection of this endangered plant species. About 52actinomycete isolates were isolated usingserial dilution and spread-plate techniques. The antagonistic activity of actinomycete isolates was assayed using an agar block method against 4 typical bacterial and fungal species. The protease and amylase activities were detected by the transparent plate method. The results show that 11isolates showed antibacterial activity and 20 isolates showed protease and amylaseactivity. In conclusion, rhizosphere soil of PaeconiaJishanensis possesses a high diversity of actinomycetes and serves as a natural source of bioactive compound-producing actinomycetes.

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