Abstract:
Genomic DNA of 160 first priority parentals from the Sugar Regulatory Administration were
extracted and subjected to genetic diversity analysis using 14 polymorphic SSR markers, two of
which were developed from a previous study. Statistical indices of the genetic diversity are: HE =
0.998926 and PIC = 0.84, showing high genetic diversity and efficacy of the markers used. Three
different clusters (I, II,III) were generated at dissimilarity coefficient 0.68. Cluster I had 10
varieties, mostly VMC and other non-Phil varieties. Sixteen varieties, mostly Phil 88- to Phil 90-
varieties were grouped in Cluster II. Cluster III had 134 varieties, mostly Phil 58- to Phil 2005-
series. Principal Component Analysis (PCA) also showed that alleles from the 160 SRA first
priority parentals were widely distributed and grouped accordingly based on their initial
clustering in the dendrogram. |
