Abstract:
Spent coffee grounds (SCG) obtained after preparing different coffee beverages, considered as a
waste, is an excellent source of polyphenols with prominent antioxidative activity. The present
study is focused on the SCG obtained as a result of preparation of black - so called "Turkish”
coffee. The effects of polyphenol-rich black coffee SCG extracts on platelets activation and
platelet-monocytes and platelet-neutrophils aggregate formation in vitro were investigated. The
antiplatelet activity of defatted black SCG extracts were measured using flow cytometry, in
blood samples from healthy subjects. The effects of investigated polyphenol-rich extracts on Pselectin
and GPIIbIIIa expression on platelets and their further aggregation with monocytes and
neutrophils were assessed in samples activated by adenosine diphosphate as platelet agonist. All
tested extracts induced the significant, dose-dependent inhibitory effect both on P-selectin and
GPIIb/IIIa receptors (15%). Statistically significant and dose-dependent inhibitory effect was
recorded on platelet aggregation with monocytes (20%) and neutrophils (22%). These results
suggest that black coffee SCG can be recommended as a valuable and low-cost source of
polyphenols with observed antiplatelet activity that could be used as an ingredient in functional
food.
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