Authors:
L. Aboudlaou
, B. Ouzyou
, M. Agnaou
, A. Hikmat
, A. Laalaoui
, A. Banaoui
, I. Ait ICHou
, A. Kaaya |
Abstract:
The aim of this work is to study and test, as biomarker of pesticide contamination, the
acetylcholinestersae activity (AChE) in the honeybee Apis mellifera. Kinetic parameters, organ
distribution and the response of the AChE to field and laboratory exposure to pesticide were
studied. The extraction of the enzyme must be carried out in Tris buffer or phosphate buffer.
AChE activity was distributed mainly in the head compared to the thorax and the abdomen. The
optimum assay temperature is 25 C while the optimal pH is 9. The apparent Km value was 0.4
mM and the apparent Vmax was 6.8 nmoles/min/mg proteins. The study of the effect of
exposure of Apis mellifera to deltamethrine and agricultural contamination were used as a
preliminary test to demonstrate the reaction of AChE to a type of pesticide used in the Souss
Massa region. Results showed an effect that depends on the exposure dose. The comparative
study of AChE activity according to sites in the Souss Massa were conducted in Houara region
(considered as a contaminated site) and Tassila (considered as a control site).The bee Apis
mellifera shows a high AChE activity in animals from contaminated sites (Houara),compared to
those recolted in the control site (Tassila) |
