Abstract:
Flies are the most important insect pest associated with poultry, where the accumulated organic
waste and favorable environmental conditions often promote rapid development of large
populations. This study aims to evaluate flies as a vector for avian viral pathogens. A total of 90
flies were collected from campus of School of Veterinary Medicine, Shiraz University, Iran.
Reverse transcription-polymerase chain reaction test using specific published primers for
Newcastle Disease virus, Avian Influenza virus, Infectious Bronchitis virus and Infectious Bursal
Disease virus was carried out to detect the viruses. The M1 gene of avian influenza and F gene of
Newcastle disease virus genes were detected from 18 and 32 separated samples respectively by
reverse transcription-polymerase chain reaction. Infectious Bronchitis and Infectious Bursal
Disease viruses were not detected from all houseflies. These results suggest that it is possible that
flies could become a mechanical transmitter of avian influenza and Newcastle disease viruses.
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