Authors:
Kodjovi Atasse Dansou kodjo, Assion Setu Mivedor, Kossikouma Djodji Adjata, Jerome Duclercq, Emmanuelle Muller And Yawovi Mawuena Dieudonne Gumedzoe |
Abstract:
Background and Objective: Climate change forces insect vectors to adapt to plants that were not their natural hosts, and this contributes to the emergence of begomoviruses. Cassava mosaic disease (CMD) caused by cassava whitefly-transmitted Begomoviruses is the main constraint on the progress of cassava production in Africa. The present study aims to examine the potential Begomoviruses infecting cassava other than those responsible for CMD.
Material and Methods: Foliar samples are collected from cassava, nine associated crops, and weeds in cassava fields across the five economic regions of Togo in 2015. PCR is performed with the degenerate primers AV494/AC1048 to amplify the coat protein gene of begomoviruses, followed by a direct sequencing.
Results: The presence of begomoviruses other than the traditional well-known ones on cassava is detected in cassava samples. Analyses of partial sequences of coat protein of ten amplicons reveal the presence of five begomovirus groups: African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), Tomato leaf curl Kumasi virus (ToLCKuV) on cassava; ToLCKuV and Tomato leaf curl Nigeria virus (ToLCNV) on tomato, and Ageratum leaf curl Cameroon virus (ALCCMV) on pepper.
Conclusion: Tomato begomoviruses ToLCKuV are then identified on cassava, and ALCCMV on pepper. This study will help understand the epidemiology related to whitefly transmissible geminiviruses. This is the first report of ToLCKuV on cassava and ALCCMV on pepper in Togo.
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