Abstract:
The aim of this study was to evaluate in vivo and in vitro antagonistic potential of five strains of Trichoderma spp. against phytopathogenic fungus Sclerotium rolfsii. Two in vitro tests were carried out, which was rated the pathogen pairing and antagonist and the inhibition of pathogen growth by volatile metabolites method. The pairing test consisted in inoculating of both pathogen and antagonist in the same petri dish and rating mycelial growth of the pathogen. In volatile metabolites test methodology was applied to overlapping plates, where we evaluated the inhibition of mycelial growth of the pathogen. In vivo test consisted of planting cowpea seeds and inoculating spores of the pathogen and antagonist, and then made biometrics. The data obtained by the pairing method showed that there was only 36% of mycelial growth of the pathogen paired with TC001, TC002 and TC003 strains compared to the control. The technique of overlapping plates showed that the TC003 and TC005 strains were the most effective in inhibiting the mycelial growth with 93.77 and 90.44% inhibition consecutively. The in vivo test showed that strains of Trichoderma spp. They were able not only to combat the pathogen, but also conferred growth promoting bean plants. |
