Authors: F. I. Abbator, U. M. Kolo, A. M. Said., Z. M. Chana,l.g. Asheikh , S. Daniel. and A. U. Sanda |
Abstract: The study was conducted to determine the effect of exogenous enzyme supplementation on rumen count, isolation and identification of bacteria in sheep fed a concentrate diet. Twenty (20) sheep weighing on average 22.65kgwere used for the study. The animals were weighed and divided into four (4) groups. Each group of five (5) animals was randomly assigned to one of the treatments in a Complete Randomized Design (CRD). Rumen liquor was collected from three (3) animals in each treatment for the rumen bacterial count. The enzyme was included at the levels of 0, 200, 400 and 600g in T1 (control), T2, T3 and T4 respectively. The result of the study showed that the bacterial species isolated and identified from the rumen liquor were four of fiber digesting bacteria (Escherichia coli, Streptococusfoecolis, Staphylococcus albus and Corynebacterium spp.) and three amylolytic bacteria (Bacillus subtilis, Klebsiella spp. and Proteus mirabilis). The rumen bacterial count differed significantly (P<0.05) among the treatments, while supplementation with exogenous enzyme had no effect (p?0.05) on the rumen pH. The result showed that sheep on concentrate diet only (T1) had significantly (p?0.05) lower bacterial count, while bacterial count of sheep on T2, T3 and T4 increased (p? 0.05) as the level of supplementation with the enzyme increases. In conclusion, the result of the study showed that supplementation with enzyme increased rumen bacterial count and had no effect on the rumen pH |