The corn root rhzosphere and 23 local microorganisms strain belonging to the Trichoderma family were isolated from other sources. From these strains, during the experiments, Trichoderma sp 4 micromiset strain was selected due to its high antifungal activity. The temperature of 24-28°? for the growth of the fungal strain of Trichoderma sp 4, the nutrient medium was determined to be optimal in the potato dextrose agar with a ?? of 5-7. Fusarium oxysporum, Verticillium dahliae and Alternaria alternate were found to exhibit active antagonistic properties against phytopathogenic fungi.