The study examined factors influencing gender participation in fish production in Ido local government area of Oyo State, Nigeria. Multi stage sampling technique was used to select 136 fish farmers through a well-structured questionnaire. Data was analyzed using descriptive statistics, Principal Component Analysis (PCA), three-points Likert scale and Tobit regression model. Result showed that more than 70% of the fish farmers were male literate person with an average age of 42 years.  Majority of the male (80.21%) and female (77.5%) fish farmers were married while more than 60% had no access to credit facility and fishing licences. Male fish farmers always participated in the selection of fish (96.92%), pond construction (99.26%) and purchasing of inputs (98.53%) activities while the female rarely participate in all. Notwithstanding, 67.80% of the female fish farmers always participated in feed formulation activities while 97.79% of them sometimes participated in fish feeding. Factors such as making fish farming as the primary occupation, higher fish income and access to equipment were significant and positively influenced participation in fish farming production among the male fish farmers while the variable factors such as cultural beliefs and social responsibilities had negative contribution toward the participation of the female gender in fish production. Educational level (β= 0.020, p<0.05), access to credit (β= 3.874, p<0.01) and fishing licences (β= 0.270, p<0.05) positively influenced both fish farmer’s gender participation in fish production. It is necessary for policy makers to establish a local fish forum or group so as to offer educational training regarding those cultural norms that prevent women’s full participation in fish production. Encouraging and supporting income diversification among fish farmers could strengthen fish farmers to reinvest secondary income into fish production.