Water is one of the most important renewable resources to living organisms. Water pollution remains a serious global contemporary environmental issue that affects every living thing. This study was undertaken to analyze the physicochemical and bacteriological quality of well water in Oju LGA. It also looked at the seasonal variations of these parameters and compared their values with that of the World Health Organization (WHO) and National Standard for Drinking Water Quality (NSDWQ) standards. A total of 40 well water were sampled randomly from five different locations and collected into clean plastics and sterile bottles. These samples were immediately transported to the laboratory for analysis. Standard methods were used in carrying out the physicochemical parameters, while bacteriological analysis was carried out using pour plate method. Data were analyzed statistically using one-way ANOVA, Descriptive and T-test at p < 0.05 significant level. Results from this study shows that physicochemical properties of water samples varied across locations (A-E) with significant differences observed for pH (p = 0.001), TDS (P < 0.05), temperature (p = 0.05), EC (p =0.027). The bacteriological analysis revealed a higher concentration of Enterobacteria spp., Shigella spp and Bacillus spp during the rainy season (1.20 ± 0.84, 0. 60 ± 0.89 and 2.20 ± 0.450) as compared to the dry season (0.80 ±0.84, 0.40 ± 0.55 and 1.80 ± 0.84) respectively. Seasonal variation in TVC and TCC were significantly different (P < 0.001). In a whole, location C was highly contaminated as compared to other locations. In conclusion, contamination of well water is traceable to poor sanitation practice and incessant use of agrochemical. This study recommends that further studies should be conducted to compare the quality of boreholes with well water to ascertain its quality for human consumption.