Knowledge of the morphological diversity of a germplasm collection is fundamental for genebank managers and plant breeders. The main objective of the present work was to characterize 33 landraces of carrot from 13 different regions of Tunisia, based on 34 agromorphological characters related to leaves and roots. The Shannon-Weaver Diversity (H’) index was used to study the phenotypic diversity. The estimated H’ ranged from 0.19 for core colour compared to cortex colour (RCCCC) to 0.99 for leaf division (LD). Analysis of variance revealed significant differences among landraces for all quantitative characters. Stepwise multivariate analyses were carried out to identify the useful characters that can distinguish among landraces. This study showed that qualitative characters were the best for the delimitation of landraces in this collection. Cluster analysis permitted the subdivision of carrot collection into four distinct groups independent of their geographic distribution. This information will be helpful to curators in the management and improvement of carrot germplasm in Tunisia