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ISSN : 2456-8643

Title:
METHICILLIN-RESISTANT STAPHYLOCOCCI FROM DOGS AND CATS WITH DERMATOLOGIC PROBLEMS

Authors:
Belgi DIREN SIGIRCI ,Turkey

Abstract:
Methicillin-resistant staphylococci, which are commonly found worldwide, known as one of the most important hospital infections and it is a problem in our country. The aim of the study was to examine the distribution of methicillin-resistant staphylococci (MRS) from dogs and cats with dermatologic problems. Pre-moistened swab samples were collected from skin lesions of 69 dogs and 23 cats, which were brought to the Microbiology Department for mycological examination during one year. Swabs were cultured onto Mannitol Salt Agar (supplemented with 5 g/ml oxacillin) plates and incubated at 37 C for 24 hours. After incubation, identification of the methicillin-resistant isolates was performed by conventional methods and API Staph-Ident system. Methicillin resistance was detected phenotypically by the disc diffusion method and following genotypically by PCR for the detection of the mecA gene. Antibiotic susceptibility test was made according to CLSI standard by using penicillin G, amoxicillin/clavulanic acid, erythromycin, chloramphenicol, cephalothin, azithromycin, trimethoprim /sulphamethoxazole. 4.35 % of dogs with skin problems and 4.35 % of cats with skin problems are MRS positive. 66.7% of MRS positive dogs have methicillin-resistant S. pseudintermedius. Using empirical antimicrobials should be avoided, and veterinarians should consider MRS not only for the patient but also for epidemiological implications.

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