Title: BIO-CONTROL OF LENTIL WILT DISEASE BY TRICHODERMA HARZIANUM
Authors: FerdousAkter,Md. Giush Uddin Ahmed
, Md. Jahanggir Alam
, Nazma Begum ,Bangladesh
Abstract: Fusarium caused vascular wilt of lentilisaseriousbiotic threat, sometimes lowered the yield
severely as pathogen resides the field always for their wide mode of nutrition. Trichoderma
initiated bio-control was well accepted for their beneficial arsenal rather chemical hazard.
Assessment of antagonism of T. harzianum against F. oxysporum by in-vitro and ex-vitroto
reduce lentil wilt was the goal of this study. In vitro antagonistic study shows that T.
harzianumIMI 392432 restricted the growth of F. oxysporum most in both method of dual
culture but inhibition was highest (mean PIRG- 73%) in method -2 wherein test fungi paired in
two opposite poles on PDA plate at 28 2°C temperature. 100% Trichoderma metabolites
containing culture filtratein ThFDA media totally prevents pathogens growth. It is assumed that
antibiotic compound produced by the T. harzianumturns poison substrate for respective
pathogen. Tricho-compost treated lentil plant shown maximum reduction of wilt incidence
compared to T. harzianumspore suspension and other combinations in ex-vitropot study. Wilt
disease appeared most (63%)in combining treatment of conventional compost + Fusariumspore
suspension. Population density of both test fungi in conventional compost is high and maximum
population of T.harzianum found in conventional compost +Tricho-compost application.