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ISSN : 2456-8643

Title:
BIO-CONTROL OF LENTIL WILT DISEASE BY TRICHODERMA HARZIANUM

Authors:
FerdousAkter,Md. Giush Uddin Ahmed ,M. FirozAlam , Md. Jahanggir Alam , Nazma Begum ,Bangladesh

Abstract:
Fusarium caused vascular wilt of lentilisaseriousbiotic threat, sometimes lowered the yield severely as pathogen resides the field always for their wide mode of nutrition. Trichoderma initiated bio-control was well accepted for their beneficial arsenal rather chemical hazard. Assessment of antagonism of T. harzianum against F. oxysporum by in-vitro and ex-vitroto reduce lentil wilt was the goal of this study. In vitro antagonistic study shows that T. harzianumIMI 392432 restricted the growth of F. oxysporum most in both method of dual culture but inhibition was highest (mean PIRG- 73%) in method -2 wherein test fungi paired in two opposite poles on PDA plate at 28 2C temperature. 100% Trichoderma metabolites containing culture filtratein ThFDA media totally prevents pathogens growth. It is assumed that antibiotic compound produced by the T. harzianumturns poison substrate for respective pathogen. Tricho-compost treated lentil plant shown maximum reduction of wilt incidence compared to T. harzianumspore suspension and other combinations in ex-vitropot study. Wilt disease appeared most (63%)in combining treatment of conventional compost + Fusariumspore suspension. Population density of both test fungi in conventional compost is high and maximum population of T.harzianum found in conventional compost +Tricho-compost application.

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