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ISSN : 2456-8643

Title:
IN VITRO STERILIZATION METHOD OF THE BANYUWANGIS LOCAL RED DURIAN LEAF EX PLANTS TO SEVERAL COMBINATION TYPES OF STERILIZATION MATERIALS

Authors:
Yusmia Widiastuti, Khoirul Bariyyah, Putri Istianingrum, Didik Puji Restanto and Sri Hartatik ,Indonesia

Abstract:
This research aimed to determine the most appropriate explant sterilization material in in vitro propagation of red durian. By doing so, it may be able to determine the successful research and become the research focus in obtaining the standard method of sterilization. Furthermore, this research was held in January to May 2018 at the Tissue Culture Laboratory of Universitas Jember. The planting material used was red durian Sunrise of Java (SOJ) plant originated from Songgon, Banyuwangi, East Java, Indonesia. Specifically, the planting material used was young foliage at the axillary bud, which was the result of one year mini grafting. This research used 7 sterilization methods (P1 P7), whereas each of them was processed by combining several sterilization materials such as Clorox, beta dine, detergent, alcohol 70%, bactericide, fungicide, tween 20, and dettol. Moreover, the sterilized explant was planted in MS 0 media and incubated in a 20-25 C room with the light intensity of 1000 2000 lux. After that, the researches observed it for 10 14 days. The parameter of such observation includes: living explants percentage (%), contaminated explants percentage (%), and browning explants (%). The result of this research reveals that, among the others, P7 is the best treatment since contaminated explants were not found. However, such treatment did not have any living explants because 100% of them were browning.

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