Abstract:
Efficiency of commercial farms can be improved through the application of developed
technologies such as the use of microsatellite repeats or simple sequence repeats (SSRs) as
genetic markers in plant species. This study developed sets of simple sequence repeat markers
(SSRs) from Phil 97-3933 variety, a cultivar known to be highly resistant to sugarcane smut and
downy mildew. For the library construction, genomic DNA of Phil 97-3933 was extracted and
was digested using methyl-sensitive restriction enzymes PstI and AatII, with six base pair
recognition sites. Two hundred sequences were obtained from which 27 contained SSR. A total
of 27 SSR primers were developed from sugarcane CV Phil 97-3933 using BatchPrimer3 [1].
SGS P20 had similar sequence identity to Saccharum hybrid cultivar R570 clone BAC 227O17,
while SGS P141 had similar sequence identity to S. officinarum clone LA154P24. Other SSR
primers that returned BLASTn similar sequence identities are SGS P131 (Sorghum hypothetical
protein), SGS P76 (S. officinarum clone LA34B02), SGS P112 (Saccharum hybrid BAC
235G19), SGS P125 (Sorghum hypothetical protein), and SGS P139 (Sorghum voucher BTx623
locus pSB1123). The rest of the primers identified did not return any BLASTn result. Phil 97-
3933 is a cultivar known to be highly resistant to sugarcane smut. Sugarcane smut caused by
Sporisorium scitamineum is one of the most serious diseases of sugarcane [2] and has been a
long-standing problem in the Philippines. Constructing a genomic library from Phil 97-3933, and
developing microsatellite markers from it is a start. Screening and evaluating germplasm
collections with SSR markers developed from this local variety could both optimize and
facilitate the breeding process in the country. |
