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ISSN : 2456-8643

Title:
POLYPHENOLS FROM SPENT BLACK COFFEE GROUND MODULATE PLATELET ACTIVITY

Authors:
Marija RANIC, Aleksandra KONIC-RISTIC , Suzana DIMITRIJEVIC BRANKOVIC , Tamara POPOVIC and Maria GLIBETIC

Abstract:
Spent coffee grounds (SCG) obtained after preparing different coffee beverages, considered as a waste, is an excellent source of polyphenols with prominent antioxidative activity. The present study is focused on the SCG obtained as a result of preparation of black - so called "Turkish” coffee. The effects of polyphenol-rich black coffee SCG extracts on platelets activation and platelet-monocytes and platelet-neutrophils aggregate formation in vitro were investigated. The antiplatelet activity of defatted black SCG extracts were measured using flow cytometry, in blood samples from healthy subjects. The effects of investigated polyphenol-rich extracts on Pselectin and GPIIbIIIa expression on platelets and their further aggregation with monocytes and neutrophils were assessed in samples activated by adenosine diphosphate as platelet agonist. All tested extracts induced the significant, dose-dependent inhibitory effect both on P-selectin and GPIIb/IIIa receptors (15%). Statistically significant and dose-dependent inhibitory effect was recorded on platelet aggregation with monocytes (20%) and neutrophils (22%). These results suggest that black coffee SCG can be recommended as a valuable and low-cost source of polyphenols with observed antiplatelet activity that could be used as an ingredient in functional food.

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